Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

v CHAPTER 1. GENERAL INTRODUCTION 1 Dissertation Organization 1 Cell Imaging 1 Chemiluminescence Detection 8 ATP 12 Our Goal 16 References 16 CHAPTER 2. REAL-TIME MONITORING OF SINGLE BACTERIUM LYSIS AND LEAKAGE EVENTS BY CHEMILUMINESCENCE MICROSCOPY 24 Abstract 24 Introduction 25 Experimental Section 28 Results and Discussion 32 Conclusions and Prospects 39 Acknowledgements 40 References 40 Figure Captions 43 CHAPTER 3. QUANTITATIVE IMAGING OF GENE EXPRESSION IN INDIVIDUAL BACTERIAL CELLS BY CHEMILUMINESCENCE 53 Abstract 53 Introduction 54 Experimental Section 56 Results and Discussion 60 Conclusions 69 Acknowledgements 69 References 70 Figure Captions 74 CHAPTER 4. IMAGING LOCALIZED ASTROCYTE ATP RELEASE WITH FIREFLY LUCIFERASE IMMOBILIZED BEADS ATTACHED ON CELL SURFACE 84 Abstract 84 Introduction 85